Description
Human BRCA2 Recombinant Protein (N-His)
Human BRCA2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys2308-His2537, with an N-terminal His tag. Predicted molecular weight: 28.49 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Breast cancer type 2 susceptibility protein (BRCA2) is a ~384 kDa protein. Involved in double-strand break repair and/or homologous recombination. For research use only.
Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation.
| Product Name: | Human BRCA2 Recombinant Protein (N-His) |
| SKU: | GXRP01742 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BRCA2, FANCD1, FACD, Fanconi anemia group D1 protein, Breast cancer type 2 susceptibility protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys2308-His2537 |
| Calculated MW: | 28.49 kDa |
| UniProt: | P51587 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
