Description
Human BRD3 Recombinant Protein (N-His)
Human BRD3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys35-Pro141, with an N-terminal His tag. Predicted molecular weight: 15.11 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Bromodomain-containing protein 3 is a ~79 kDa protein. Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures. For research use only.
Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4. Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression. Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets.
| Product Name: | Human BRD3 Recombinant Protein (N-His) |
| SKU: | GXRP12106 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BRD3, Bromodomain-containing protein 3, KIAA0043, RING3-like protein, RING3L |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys35-Pro141 |
| Calculated MW: | 15.11 kDa |
| UniProt: | Q15059 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
