The Human C1INH (Complement 1 Inhibitor) ELISA Kit is a specialized assay designed for the precise quantification of Complement 1 Inhibitor levels in human samples. C1INH plays a vital role in regulating the body's complement system, a key component of the immune response. By inhibiting the activation of complement proteins, C1INH helps maintain immune balance and prevent excessive inflammation. This ELISA kit enables accurate measurement of C1INH levels, offering insights into the immune response, inflammatory processes, and disorders related to complement system dysregulation.
Accurate quantification of C1INH is essential for understanding its role in immune homeostasis, inflammatory diseases, and autoimmune conditions. The C1INH ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. With robust performance and user-friendly protocols, this kit is suitable for a wide range of research and clinical applications. Trust Assay Genie's C1INH ELISA Kit for precise and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human C1INH (Complement 1 Inhibitor) ELISA Kit
SKU:
AEES00088
Target:
Human C1INH (Complement 1 Inhibitor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1INH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1INH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1INH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C1INH. You can calculate the concentration of Human C1INH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-102
99-109
Average (%)
100
99
105
1:4
Range (%)
99-109
87-98
92-102
Average (%)
103
91
97
1:8
Range (%)
95-110
87-99
88-104
Average (%)
103
94
96
1:16
Range (%)
86-99
92-105
84-94
Average (%)
91
98
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-94
90
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
96-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.93
201.44
837.57
90.23
202.24
863.52
Standard deviation
5.01
9.75
34.09
5.38
9.73
34.63
C V (%)
5.22
4.84
4.07
5.96
4.81
4.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C1INH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C1INH in samples. No significant cross-reactivity or interference between Human C1INH and analogues was observed.
