Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit
The Human C1Q and Tumor Necrosis Factor-Related Protein 1 (C1QTNF1) ELISA Kit is specifically designed for the accurate and reliable detection of C1QTNF1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.C1QTNF1 is a key protein involved in regulating inflammation and metabolism, playing a crucial role in conditions such as obesity, diabetes, and cardiovascular diseases.
By measuring C1QTNF1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.With its easy-to-use format and precise results, the Human C1QTNF1 ELISA Kit is an essential tool for researchers studying the role of C1QTNF1 in various disease processes and exploring potential treatment options.
Product Name:
Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit
Product Code:
HUDL00371
Size:
96 Assays
Target:
C1QTNF1
Synonyms:
GIP, CTRP1, ZSIG37, G Protein Coupled Receptor Interacting Protein
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
