The Human CA19-9 (Carbohydrate Antigen 19-9) ELISA Kit is specifically designed for the quantitative measurement of CA19-9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and precise results for various research applications.CA19-9 is a tumor-associated antigen often overexpressed in various cancers, particularly pancreatic, colorectal, and gastric cancers. Monitoring CA19-9 levels can aid in early cancer detection, prognosis, and treatment monitoring, making it a valuable biomarker in oncology research.With its reliable performance, the Human CA19-9 ELISA Kit is a valuable tool for researchers studying cancer biology, diagnostics, and therapy development. Get accurate and reproducible results with this high-quality kit from Assay Genie.
Product Name:
Human CA19-9 (Carbohydrate antigen19-9) ELISA Kit
SKU:
HUES01800
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 IU/mL
Detection range:
6.25-400 IU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
