Description
Human CA5A Recombinant Protein (N-His)
Human CA5A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln79-Ser305, with an N-terminal His tag. Predicted molecular weight: 27.98 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Carbonic anhydrase 5A, mitochondrial (CA5A) is a ~34 kDa protein. Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3. For research use only.
Mitochondria are impermeable to HCO3, and thus this intramitochondrial carbonic anhydrase is pivotal in providing HCO3 for multiple mitochondrial enzymes that catalyze the formation of essential metabolites of intermediary metabolism in the urea and Krebs cycles. 1. van Karnebeek, CD. et al. (2014) American journal of human genetics 94, 453-61. PMID: 24530203 2. Nagao, Y. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7623-7. PMID: 8356065
| Product Name: | Human CA5A Recombinant Protein (N-His) |
| SKU: | GXRP08719 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Carbonic anhydrase 5A mitochondrial, EC:4.2.1.1, Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA, CA5A |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln79-Ser305 |
| Calculated MW: | 27.98 kDa |
| UniProt: | P35218 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
