Description
Human CAD Recombinant Protein (N-His)
Human CAD Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu1561-Lys1811, with an N-terminal His tag. Predicted molecular weight: 30.64 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Multifunctional protein CAD is a ~242 kDa protein. Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). For research use only.
The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate.
| Product Name: | Human CAD Recombinant Protein (N-His) |
| SKU: | GXRP11094 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Ammonium-dependent carbamoyl phosphate synthase, Aspartate carbamoyltransferase, CAD, CPS, CPSase, Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase, Dihydroorotase, EC:2.1.3.2, EC:3.5.1.2, EC:3.5.2.3, EC:6.3.4.16, EC:6.3.5.5, GATase, GLNase, Glutamine amidotransferase, Glutamine-dependent carbamoyl phosphate synthase, Multifunctional protein CAD |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu1561-Lys1811 |
| Calculated MW: | 30.64 kDa |
| UniProt: | P27708 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
