Human Calcium-Transporting ATPase Type 2C Member 1 (ATP2C1) ELISA Kit
The Human Calcium Transporting ATPase Type 2C Member 1 (ATP2C1) ELISA Kit is a powerful tool for accurately measuring ATP2C1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.ATP2C1 is a key protein involved in calcium transport within cells, playing a crucial role in cellular functions such as muscle contraction, hormone secretion, and nerve signaling.
Dysregulation of ATP2C1 has been linked to diseases like Darier's disease and Hailey-Hailey disease, highlighting its importance as a biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human Calcium Transporting ATPase Type 2C Member 1 (ATP2C1) ELISA Kit is an indispensable tool for researchers looking to investigate the role of ATP2C1 in health and disease, with the potential to advance our understanding of cellular calcium homeostasis and its implications for human health.
Product Name:
Human Calcium-Transporting ATPase Type 2C Member 1 (ATP2C1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
