The Human CAM (Calmodulin) CLIA Kit is a reliable and accurate tool for detecting calmodulin levels in human samples. Calmodulin is a key calcium-binding protein that plays a vital role in regulating various cellular processes such as muscle contraction, cell division, and neurotransmitter release.This kit offers high sensitivity and specificity, ensuring precise and reproducible results for researchers working in the fields of cell biology, neuroscience, and drug development.
By measuring calmodulin levels, scientists can gain valuable insights into the mechanisms underlying diseases such as Alzheimer's, Parkinson's, and heart disease.With its ease of use and robust performance, the Human CAM CLIA Kit is an essential tool for studying the role of calmodulin in health and disease, ultimately leading to the development of novel therapeutic interventions.
Product Name:
Human CAM (Calmodulin) CLIA Kit
SKU:
HUES00413
Target:
Human CAM (Calmodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CAM. You can calculate the concentration of Human CAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
94-106
96-108
Average (%)
105
100
103
1:4
Range (%)
93-106
101-116
103-117
Average (%)
99
109
109
1:8
Range (%)
101-116
102-117
99-115
Average (%)
108
109
106
1:16
Range (%)
99-114
101-119
93-107
Average (%)
108
110
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.64
305.91
666.67
92.39
277.25
711.63
Standard deviation
10.33
25.79
59.53
10.55
20.9
70.1
C V (%)
11.27
8.43
8.93
11.42
7.54
9.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CAM in samples. No significant cross-reactivity or interference between Human CAM and analogues was observed.
