Human Carnitine Palmitoyltransferase 1B, Muscle (CPT1B) ELISA Kit
The Human CPT1B (Carnitine Palmitoyltransferase 1B Muscle) ELISA Kit is a specialized assay designed for the precise measurement of CPT1B levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CPT1B is a key enzyme involved in fatty acid oxidation and energy metabolism in muscle cells, playing a crucial role in maintaining energy homeostasis. Dysregulation of CPT1B has been implicated in various metabolic disorders, making it a valuable biomarker for studying metabolic diseases and potential therapeutic interventions.
By utilizing the Human CPT1B ELISA Kit, researchers can gain valuable insights into the role of CPT1B in metabolic processes and its potential implications for diseases such as obesity, diabetes, and cardiovascular disorders. This kit provides a reliable and efficient tool for investigating the functions of CPT1B, paving the way for advancements in metabolic research and drug development.
Product Name:
Human Carnitine Palmitoyltransferase 1B, Muscle (CPT1B) ELISA Kit
Product Code:
HUDL00662
Size:
96 Assays
Target:
CPT1B
Synonyms:
CPT1-M, Carnitine palmitoyltransferase I-like protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
