Human CD135/FLT3 (IMC-EB10) Antibody is a research-grade biosimilar monoclonal antibody. It is a human IgG antibody. Reactivity: Human. Supplied as a liquid in 0.01M PBS pH 7.4. Applications: ELISA, flow cytometry, functional assays and in vivo research. Endotoxin: < 10 EU/mg. Purity: >95% purity as determined by SDS-PAGE. For research use only.
Receptor-type tyrosine-protein kinase FLT3 (CD135) is a ~112 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B.
Product Name:
Human CD135/FLT3 (IMC-EB10) Antibody
SKU:
GXBS01311
Size:
100 µg, 1 mg
Antibody Type:
Monoclonal Antibody
Clone:
IMC-EB10
Target:
FLT3
Isotype:
IgG
Host Species:
Human
Reactivity:
Human
Applications:
ELISA, FACS, Functional assay, Research in vivo
Expression Host:
Mammalian cells
Synonyms:
IMC-EB10
Biological Activity:
IMC-EB10 (10 ug/mL, 1 h) reduces FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK in Hb1119 and SEM-K2 cells, but activates them in REH and RS411 cells [1]. IMC-EB10 (10 ug/mL, 1 h) activates FLT3 and downstream Akt in ALLs [1]. IMC-EB10 (0.4-200 nM, 1 h) inhibits FLT3-Fc-induced phosphorylation of wild-type FLT3 and ligand-independent constitutive phosphorylation of ITD-mutant FLT3 in EOL-1, EM3, BaF3-ITD and MV4;11 cells [4]. IMC-EB10 (0.4-200 nM, 1 h) inhibits FLT3-Fc-induced phosphorylation of MAPK, AKT and Stat5 in EOL-1, EM3, BaF3-ITD and MV4;11 cells [4]. IMC-EB10 (1.5-100 nM, 1 h) inhibits proliferation of FLT3-Fc-induced proliferation of EOL-1 and BaF3-ITD cells [4]. Western Blot Analysis [1] Cell Line: Hb1119, SEM-K2 cells Concentration: 10 ug/mL Incubation Time: 1 h Result: Reduced FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK. Western Blot Analysis [1] Cell Line: REH, RS411 cells Concentration: 10 ug/mL Incubation Time: 1 h Result: Activated FLT3 and phosphorylation of downstream signaling proteins STAT5, Akt, and MAPK. Western Blot Analysis [1] Cell Line: ALLs Concentration: 10 ug/mL Incubation Time: 1 h Result: Activated FLT3 and downstream Akt. . Blocked the phosphorylation and constitutive phosphorylation of MAPK, inhibited FL-induced phosphorylation of AKT and Stat5 phosphorylation in EOL-1 and BaF3-ITD cells. Parmacokinetics Species Dose Route T 1/2 AUC 0-inf Mice [2] 20 mg/kg i.p. 96 h 38327 ug·h/mL IMC-EB10 (400 ug/10 mg/kg, i.p.
Purity:
>95% purity as determined by SDS-PAGE.
Endotoxin Level:
< 10 EU/mg
Formulation:
0.01M PBS pH 7.4
State of Matter:
Liquid
Regulatory Status:
For research use only. Not suitable for clinical or therapeutic use.
Product Preparation:
Protein A/G purified from cell culture supernatant.
Storage and Handling:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
