Human CD198/CCR8 (HFB101110) Antibody is a research-grade biosimilar monoclonal antibody. It is a human IgG antibody. Reactivity: Human. Supplied as a liquid in 0.01M PBS pH 7.4. Applications: ELISA, flow cytometry, functional assays and in vivo research. Endotoxin: < 10 EU/mg. Purity: >95% purity as determined by SDS-PAGE. For research use only.
C-C chemokine receptor type 8 (CD198/CCR8) is a ~40 kDa protein. G protein-coupled receptor that can bind a variety of chemokines, such as CCL1, CCL8, CCL16, CCL18. Regulates monocyte and eosinophil chemotaxis. Undergoes internalization upon CCL18 binding, leading to induced migration and calcium flux of highly polarized Th2 cells. In microglial cells, promotes phagocytosis with CCL18 through NF-kappa-B and Src signaling pathways. Stimulation of the CCL1-CCR8 signaling axis protects the gut from acute intestinal damage.
Product Name:
Human CD198/CCR8 (HFB101110) Antibody
SKU:
GXBS00983
Size:
100 µg, 1 mg
Antibody Type:
Monoclonal Antibody
Clone:
HFB101110
Target:
CKR-L1
Isotype:
IgG
Host Species:
Human
Reactivity:
Human
Applications:
ELISA, FACS, Functional assay, Research in vivo
Expression Host:
Mammalian cells
Synonyms:
HFB101110
Biological Activity:
HFB101110 (0.01-10 nM) dose-dependently inhibits hCCL1-induced chemotaxis of CCR8 + cells [1]. HFB101110 (10 ug/mL) completely inhibits hCCL1-induced calcium flux in CCR8 + cells [1]. HFB101110 (0.3-300 ug/mL) does not induce significant cytokine release from healthy human PBMCs at concentrations up to 300 ug/mL [1]. HFB101110 exerts potent single-agent antitumor activity in a human CCR8 knock-in mouse model via depletion of intratumoral regulatory T cells [2]. HFB101110 is well-tolerated in wild-type mice [2]. HFB101110 is well-tolerated in cynomolgus monkeys [2].
Purity:
>95% purity as determined by SDS-PAGE.
Endotoxin Level:
< 10 EU/mg
Formulation:
0.01M PBS pH 7.4
State of Matter:
Liquid
Regulatory Status:
For research use only. Not suitable for clinical or therapeutic use.
Product Preparation:
Protein A/G purified from cell culture supernatant.
Storage and Handling:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
