Description
Human CD247/CD3Z Recombinant Protein (N-GST)
Human CD247/CD3Z Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Arg52-Arg164, with an N-terminal GST tag. Predicted molecular weight: 39.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. T-cell surface glycoprotein CD3 zeta chain (CD247/CD3Z) is a ~18 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. For research use only.
When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.
| Product Name: | Human CD247/CD3Z Recombinant Protein (N-GST) |
| SKU: | GXRP01178 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CD3Z, CD247, TCRZ, T-cell receptor T3 zeta chain, T3Z, T-cell surface glycoprotein CD3 zeta chain |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Arg52-Arg164 |
| Calculated MW: | 39.85 kDa |
| UniProt: | P20963 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
