Human CD30/TNFRSF8 (HeFi-1) Antibody is a research-grade biosimilar monoclonal antibody. It is a human IgG antibody. Reactivity: Human. Supplied as a liquid in 0.01M PBS pH 7.4. Applications: ELISA, flow cytometry, functional assays and in vivo research. Endotoxin: < 10 EU/mg. Purity: >95% purity as determined by SDS-PAGE. For research use only.
Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris).
Product Name:
Human CD30/TNFRSF8 (HeFi-1) Antibody
SKU:
GXBS01048
Size:
100 µg, 1 mg
Antibody Type:
Monoclonal Antibody
Clone:
HeFi-1
Target:
CD30L receptor
Isotype:
IgG
Host Species:
Human
Reactivity:
Human
Applications:
ELISA, FACS, Functional assay, Research in vivo
Expression Host:
Mammalian cells
Synonyms:
HeFi-1, HeFi1, HeFi1
Biological Activity:
HeFi-1 (0.1 ng/uL, 1 h) can bind specifically to Karpas299 cells with a binding rate of over 90 % [1]. HeFi-1 (20 ug/mL, 48 h) can significantly inhibit the proliferation of Karpas299 cells and induces G1 phase blockade [1]. HeFi-1 (20 ug/mL, 12-24 h) can significantly induce apoptosis in eosinophil [2]. Cell Proliferation Assay [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Significantly inhibited cell proliferation with an inhibition rate of 40%, superior to Daclizumab (). Cell Cycle Analysis [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Induced G1 phase arrest and a decrease in S phase cells. HeFi-1 (100 ug, i.v., once weekly, for 4 weeks) can effectively prolong survival in SCID/NOD wild-type and FcRγ -/- mice bearing Karpas299 lymphoma [1].
Purity:
>95% purity as determined by SDS-PAGE.
Endotoxin Level:
< 10 EU/mg
Formulation:
0.01M PBS pH 7.4
State of Matter:
Liquid
Regulatory Status:
For research use only. Not suitable for clinical or therapeutic use.
Product Preparation:
Protein A/G purified from cell culture supernatant.
Storage and Handling:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
