Description
Human CD301/CLEC10A Recombinant Protein (N-His)
Human CD301/CLEC10A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr71-Met239, with an N-terminal His tag. Predicted molecular weight: 21.29 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. C-type lectin domain family 10 member A (CD301/CLEC10A) is a ~35 kDa protein. C-type lectin receptor involved in recognition of N-acetylgalactosamine (GalNAc)-terminated glycans by myeloid antigen presenting cells (APCs). For research use only.
Binds in a Ca(2+)-dependent manner to alpha- and beta-linked GalNAc residues on glycoprotein and glycolipid antigens, including alphaGalNAc- and Galbeta1->3GalNAc-O-Ser/Thr also known as Tn and T antigens, LacdiNAc epitope GalNAcbeta1->4GlcNAc and its derivative GalNAcbeta1->4-(Fucalpha1->3)GlcNAc, O-linked core 5 and 6 glycans, and GM2 and GD2 gangliosides. Acts as a signaling receptor at the interface of APC-T cell interactions. On immature dendritic cells, recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and downregulates PTRPN/CD45 phosphatase activity with an impact on T cell activation threshold, cytokine production and proliferation. Modulates dendritic cell maturation toward a tolerogenic phenotype leading to generation of regulatory CD4-positive T cell subset with immune suppressive functions. 1. van Vliet, SJ. et al. (2005) International immunology 17, 661-9. PMID: 15802303 2. van Vliet, SJ. et al. (2006) Nature immunology 7, 1200-8. PMID: 16998493 3. van Vliet, SJ. et al. (2007) European journal of immunology 37, 2075-81. PMID: 17616966 4. Li, D. et al. (2012) The Journal of experimental medicine 209, 109-21. PMID: 22213806 5. Gabba, A. et al. (2021) Biochemistry 60, 1327-1336. PMID: 33724805 6. Suzuki, N. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 128-35. PMID: 8598452 7. Mortezai, N. et al. (2013) Glycobiology 23, 844-52. PMID: 23507963
| Product Name: | Human CD301/CLEC10A Recombinant Protein (N-His) |
| SKU: | GXRP02540 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CD301, Macrophage lectin 2, C-type lectin domain family 10 member A, CLECSF13, C-type lectin superfamily member 14, CLEC10A, CLECSF14, HML |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr71-Met239 |
| Calculated MW: | 21.29 kDa |
| UniProt: | Q8IUN9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
