Human CD5L (CD5 Antigen-like) Quickstep ELISA Kit The Human CD5L (CD5 Antigen-like) Quickstep ELISA Kit is designed for the quantitative detection of Human CD5L (CD5 Antigen-like) levels in various biological samples. CD5 Antigen-like is a biologically active molecule with established roles in ç»†èƒžç”Ÿç‰©å¦ pathways. It participates in key cellular and molecular processes that regulate physiological homeostasis, and dysregulation of its expression or activity has been associated with pathological states across multiple disease contexts. The precise quantification of Human CD5 Antigen-like in biological samples is critical for elucidating its mechanistic contributions to disease pathogenesis, identifying clinically relevant biomarker associations, and supporting the development of novel diagnostic tools and targeted therapeutic interventions. Assay Genie's Human CD5L (CD5 Antigen-like) Quickstep ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 0.09 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human CD5L (CD5 Antigen-like) Quickstep ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Overview Product Name: Human CD5L (CD5 Antigen-like) Quickstep ELISA Kit SKU: QSES145 Size: 96 Assays Detection Method: Colorimetric method, ELISA, Sandwich Assay type: Sandwich-ELISA Assay time: 1 h 30 min Sensitivity: 0.09 ng/mL Detection range: 0.16-10 ng/mL Reovery: 80%-120%
Assay Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
Kit Components Component Specification Storage Micro ELISA Plate (Dismountable) 96T: 8wells× 12strips | 48T: 8wells× 6strips | 24T: 8wells× 3strips | 96T*5: 5plates,96T -20°C, 12 months Reference Standard 96T: 2 vials | 48T/24T: 1vial | 96T*5: 10 vials -20°C, 12 months Concentrated HRP Conjugate (100×) 96T: 1vial,60μL | 48T/24T: 1vial,30μL | 96T*5: 5vials,60μL -20°C (Protect from light), 12 months Reference Standard & Sample Diluent 96T/48T/24T: 2 vials,20mL | 96T*5: 10 vials,20mL 2–8°C, 12 months HRP Conjugate Diluent 96T/48T/24T: 1vial,14mL | 96T*5: 5vials,14mL 2–8°C, 12 months Concentrated Wash Buffer(25×) 96T/48T/24T: 1vial,30mL | 96T*5: 5vials,30mL 2–8°C, 12 months Substrate Reagent 96T/48T/24T: 1vial,10mL | 96T*5: 5vials,10mL 2–8°C (Protect from light) Stop Solution 96T/48T/24T: 1vial,10mL | 96T*5: 5vials,10mL 2–8°C Plate Sealer 96T/48T/24T: 5pieces | 96T*5: 25pieces 2–8°C Technical Manual 1 copy - Certificate of Analysis 1 copy -
Properties Linearity: Serum (n=5) EDTA Plasma (n=5) 1:2 Range (%) 90-101 88-104 Average (%) 96 95 1:4 Range (%) 91-102 88-100 Average (%) 96 94 1:8 Range (%) 87-102 87-100 Average (%) 94 94 1:16 Range (%) 89-103 88-101 Average (%) 95 95
Recovery: Sample Type Range (%) Average Recovery (%) EDTA Plasma (n=8) 85-98 91
Precision: Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20.0 20.0 20.0 20.0 20.0 20.0 Mean (ng/mL) 0.53 0.9 4.8 0.56 0.83 4.52 Standard deviation 0.03 0.04 0.33 0.04 0.07 0.4 C V (%) 6.59 4.54 6.93 6.94 8.54 8.84
Additional Information Uniport ID: O43866 Sample type & Sample volume: Serum, Plasma And Other Biological Fluids; 50 μL Specificity: This kit recognizes Human CD5L in samples. No significant cross-reactivity or interference between Human CD5L and analogues was observed. Storage: 2-8℃ Research Area: 细胞生物å¦
