Recombinant Human CD74 Protein (His Tag) (RPES5985)
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens- associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.
Product Name:
Recombinant Human CD74 Protein (His Tag)
SKU:
RPES5985
Size:
100μg, 20μg, 500μg, 1mg
Species:
Human
Synonyms:
DHLAG, HLA class II histocompatibility antigen gamma chain, HLADG, Ia-GAMMA II
Tag:
C-His
Sequence:
Gln73-Met232
Expression Host:
HEK293 Cells
Accession:
P04233
Endotoxin:
< 1.0 EU/mg of the protein as determined by the LAL method
Purity:
> 95% as determined by reducing SDS-PAGE.
Calculated MW:
17.5 kDa
Observed MW:
25 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human CD74 protein Gln73-Met232, with an C-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to - 80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
