Human CD81 PharmaGenie ELISA Kit (SBRS0399)
- SKU:
- SBRS0399
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
Key Features
| Publication Ready | ISO certified and validated for reproducibility & reliability | |
| Save Time | Pre-coated 96 well plate | |
| Fully Optimized | For serum, plasma and cell culture supernatant samples |
Overview
|
Product Name: |
Human CD81 PharmaGenie ELISA |
|
Product Code: |
SBRS0399 |
|
Size: |
96T |
|
Reactivity: |
Human |
|
Application: |
This ELISA antibody pair detects human CD81. Other species not determined |
|
Sensitivity: |
0.36 ng/ml |
|
Range: |
0.36 ng/ml - 100 ng/ml |
|
Design Principle: |
Sandwich ELISA |
|
Sample Types: |
Cell Culture Supernatants, Plasma, Serum |
|
Method of Detection: |
Colorimetric |
|
Recommended Dilution: |
2 fold |
|
Storage/Stability: |
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
|
Synonyms: |
CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD antigen CD81 |
|
Uniprot: |
|
|
Gene ID: |
|
|
Gene Names: |
CD81, TAPA1, TSPAN28 |
|
PharmaGenie: |
PharmaGenie ELISA kits are highly validated and designed to exceed the needs of biopharma R&D scientists. |
Kit Components
| No. | Component |
|
1. |
Pre-Coated 96-well Strip Microplate |
|
2. |
Wash Buffer |
|
3. |
Stop Solution |
|
4. |
Assay Diluent(s) |
|
5. |
Lyophilized Standard |
|
6. |
Biotinylated Detection Antibody |
|
7. |
Streptavidin-Conjugated HRP |
|
8. |
TMB One-Step Substrate |
|
9. |
Colorimetric |
Other materials required:
The Assay Genie Human CD81 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol
*Note: Protocols are specific to each batch/lot.
For the exact instructions please follow the protocol included in your kit.
| Step | Procedure |
|
1. |
Prepare all reagents, samples and standards as instructed in the manual. |
|
2. |
Add 100 ul of standard or sample to each well. |
|
3. |
Incubate 2.5 h at RT or O/N at 4°C. |
|
4. |
Add 100 ul of prepared biotin antibody to each well. |
|
5. |
Incubate 1 h at RT. |
|
6. |
Add 100 ul of prepared Streptavidin solution to each well. |
|
7. |
Incubate 45 min at RT. |
|
8. |
Add 100 ul of TMB One-Step Substrate Reagent to each well. |
|
9. |
Incubate 30 min at RT. |
|
10. |
Add 50 ul of Stop Solution to each well. |
|
11. |
Read at 450 nm immediately. |
CD81 Background
CD81 Background
CD81 is a transmembrane protein that belongs to the tetraspanin family of proteins. It is widely expressed on various cell types including immune cells, hepatocytes, and endothelial cells. CD81 is involved in a wide range of cellular processes such as cell adhesion, migration, and signaling. In particular, it has been implicated in the regulation of immune responses, viral infection, and cancer progression.
CD81 Molecular Weight
The molecular weight of CD81 varies depending on its glycosylation state and can range from 26 to 32 kDa.
CD81 Exosome Marker
Exosomes are small vesicles secreted by cells that play a role in intercellular communication and can be found in a variety of bodily fluids including blood, urine, and saliva. CD81 is a commonly used exosome marker due to its high abundance on the surface of exosomes. The presence of CD81 on exosomes makes it a useful marker for the isolation and characterization of exosomes. In addition, CD81 has been shown to play a functional role in exosome biogenesis and cargo sorting.
CD81 Antibody
CD81 antibodies are important tools for studying the function and expression of CD81 protein in various biological samples. Our Anti-CD81 Antibody (CAB5270) is a high-quality polyclonal antibody that has been extensively validated for use in Western blot (WB) and immunohistochemistry (IHC) applications. This antibody is derived from rabbit host species and has been shown to react specifically with CD81 protein from human, mouse, and rat samples. CD81 antibodies can be used in a wide range of research applications, including the detection and quantification of CD81 protein expression, as well as functional studies to investigate the role of CD81 in cellular processes. With sizes available in 20uL, 50uL, and 100uL, our Anti-CD81 Antibody is a valuable tool for researchers looking to study CD81 protein in their experiments.
CD81 ELISA FAQs
Q: What is the CD81 ELISA kit used for?
The CD81 ELISA kit is a valuable tool for the detection and quantification of CD81 protein levels in biological samples.
Q: What samples can be used with the CD81 ELISA kit?
The CD81 ELISA kit is suitable for use with a variety of biological samples, including cell culture supernatants, plasma and serum.
Q: What is the sensitivity of the CD81 ELISA kit?
The sensitivity of the CD81 ELISA kit is dependent on the sample type and preparation method. Generally, the kit has a detection range of 0.36 ng/mL - 100 ng/mL.
Q: What is the shelf life of the CD81 ELISA kit?
The shelf life of the CD81 ELISA kit is typically 6 months when stored at 4°C.
Q: What should I do if I run into trouble using the CD81 ELISA kit?
If you encounter any issues with the performance of the CD81 ELISA kit, please contact our customer support team for assistance. We are always happy to help troubleshoot any problems you may encounter.
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