Human CDNF (Cerebral Dopamine Neurotrophic Factor) CLIA Kit
The Human CDNFD (Cerebral Dopamine Neurotrophic Factor) CLIA Kit is a highly sensitive and specific assay designed for accurate detection of CDNFD levels in human serum, plasma, and cell culture supernatants. With its reliable and reproducible results, this kit is perfect for various research applications.CDNFD is a vital neurotrophic factor that plays a key role in promoting the survival and function of dopamine-producing neurons in the brain.
Dysregulation of CDNFD has been linked to various neurodegenerative disorders such as Parkinson's disease, making it a critical biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human CDNFD CLIA Kit provides researchers with a valuable tool for studying the role of CDNFD in neurodegenerative diseases and developing new treatment strategies.
Product Name:
Human CDNF (Cerebral Dopamine Neurotrophic Factor) CLIA Kit
SKU:
HUES00244
Target:
Human CDNF (Cerebral Dopamine Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CDNF. You can calculate the concentration of Human CDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
88-100
94-110
Average (%)
104
93
100
1:4
Range (%)
90-106
92-103
87-100
Average (%)
96
98
94
1:8
Range (%)
100-116
95-112
88-101
Average (%)
109
102
95
1:16
Range (%)
91-102
86-98
103-116
Average (%)
97
91
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.31
243.06
819.76
99.4
236.04
811.69
Standard deviation
8.7
19.15
60.25
12.64
21.55
90.26
C V (%)
9.13
7.88
7.35
12.72
9.13
11.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CDNF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CDNF in samples. No significant cross-reactivity or interference between Human CDNF and analogues was observed.
