The Human CFB (Complement Factor B) ELISA Kit is a specialized assay designed to quantitatively detect CFB levels in a variety of biological samples. Complement Factor B is a crucial component of the alternative complement pathway involved in immune responses, complement activation, and the clearance of pathogens. It plays a pivotal role in bridging innate and adaptive immunity by participating in opsonization, inflammation, and immune complex clearance. Understanding CFB levels is essential for deciphering immune system function, complement activation, and disease processes. Accurate measurement of CFB is vital for investigating its role in immune responses and disease pathogenesis. With exceptional sensitivity and specificity, Assay Genie's CFB ELISA Kit ensures precise and reproducible results. Manufactured under strict quality control guidelines, this kit delivers reliable performance and user-friendly operation, making it an excellent choice for both laboratory research and clinical applications. Researchers can trust Assay Genie's CFB ELISA Kit for dependable quantitation of this essential complement factor in their studies.
Product Name:
Human CFB (Complement Factor B) ELISA Kit
SKU:
AEES00092
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
