Human CG beta (Chorionic Gonadotrophin Beta) CLIA Kit
The Human CG Beta (Chorionic Gonadotrophin Beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CG Beta levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal choice for researchers in a variety of fields.CG Beta is a hormone that plays a crucial role in pregnancy, being produced by the placenta and responsible for maintaining the corpus luteum.
Abnormal levels of CG Beta can indicate issues with pregnancy, such as ectopic pregnancy or miscarriage, making it a valuable biomarker for monitoring pregnancy health.This kit is essential for studying reproductive health and fertility issues, as well as for monitoring the progress of pregnancy and potential complications. Its high sensitivity and specificity make it a valuable tool for researchers and healthcare professionals alike.
Product Name:
Human CG beta (Chorionic Gonadotrophin Beta) CLIA Kit
SKU:
HUES00464
Target:
Human CG beta (Chorionic Gonadotrophin Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CGβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CGβ. You can calculate the concentration of Human CGβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
87-99
91-106
Average (%)
97
94
98
1:4
Range (%)
95-111
85-97
104-121
Average (%)
101
92
110
1:8
Range (%)
97-113
98-116
101-114
Average (%)
104
106
108
1:16
Range (%)
90-103
92-104
94-108
Average (%)
97
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.31
3.52
0.46
1.22
3.54
Standard deviation
0.05
0.14
0.31
0.06
0.1
0.36
C V (%)
10.0
10.69
8.81
13.04
8.2
10.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CGβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CGβ in samples. No significant cross-reactivity or interference between Human CGβ and analogues was observed.
