Human CHD8 (Chromodomain-helicase-DNA-binding protein 8) ELISA Kit
The Human CHD8 (Chromodomain Helicase DNA-Binding Protein 8) ELISA Kit is a powerful tool for detecting and quantifying CHD8 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it perfect for a variety of research applications. CHD8 is a critical protein that plays a key role in chromatin remodeling and gene expression regulation. Dysregulation of CHD8 has been linked to various diseases, including autism spectrum disorder and certain types of cancer.
Studying CHD8 levels can provide valuable insights into disease mechanisms and potential therapeutic targets. The Human CHD8 ELISA Kit from AssayGenie offers researchers a reliable and easy-to-use solution for investigating the role of CHD8 in health and disease. With its high performance and accuracy, this kit is an essential tool for advancing biomedical research and drug development efforts.
Product Name:
Human CHD8 (Chromodomain-helicase-DNA-binding protein 8) ELISA Kit
SKU:
AEFI00285
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-96%
92-103%
85-100%
EDTA Plasma (n = 5)
88-94%
85-98%
82-98%
Heparin Plasma (n = 5)
82-93%
83-98%
80-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
96
EDTA Plasma (n = 5)
86-102
92
Heparin Plasma (n = 5)
88-106
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
