Description
Human CHEK2 Recombinant Protein (N-His)
Human CHEK2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val300-Ala540, with an N-terminal His tag. Predicted molecular weight: 29.44 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Serine/threonine-protein kinase Chk2 (CHEK2) is a ~60 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. For research use only.
May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.
| Product Name: | Human CHEK2 Recombinant Protein (N-His) |
| SKU: | GXRP00345 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RAD53, CHK2 checkpoint homolog, hCds1, Serine/threonine-protein kinase Chk2, CDS1, Checkpoint kinase 2, CHEK2, Hucds1, Cds1 homolog, CHK2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val300-Ala540 |
| Calculated MW: | 29.44 kDa |
| UniProt: | O96017 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
