The Human Chemerin ELISA Kit (HUES01849) from AssayGenie is a cutting-edge research tool designed for the precise and accurate detection of chemerin levels in human samples. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Chemerin is a key protein involved in inflammation, adipogenesis, and metabolism, playing a critical role in conditions such as obesity, diabetes, and cardiovascular diseases. By measuring chemerin levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially discover novel therapeutic targets.The AssayGenie Human Chemerin ELISA Kit includes all necessary components for easy and efficient sample analysis, making it a valuable addition to any research laboratory studying chemerin-related pathways and diseases. Order your kit today and unlock the potential of your research.
Product Name:
Human CHEM (Chemerin) ELISA Kit
SKU:
HUES01849
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
