The Human Chemokine C Motif Ligand 2 (XCL2) ELISA Kit is a powerful tool for accurately measuring XCL2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.XCL2 is a chemokine that plays a crucial role in immune responses and inflammation by attracting and activating immune cells. Dysregulation of XCL2 has been associated with various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
By measuring XCL2 levels, researchers can gain valuable insights into the immune system and potentially develop new therapies for these diseases.Overall, the Human Chemokine C Motif Ligand 2 (XCL2) ELISA Kit offers a comprehensive solution for studying the role of XCL2 in health and disease, providing researchers with the tools needed to advance their understanding of immune responses and inflammatory processes.
Product Name:
Human Chemokine C-Motif Ligand 2 (XCL2) ELISA Kit
Product Code:
HUDL03139
Size:
96 Assays
Target:
XCL2
Synonyms:
SCYC2, SCM-1b, Small Inducible Cytokine Subfamily C Member 2, Cytokine SCM-1 beta, C motif chemokine 2, XC chemokine ligand 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
