Human Chitinase 3 Like Protein 2 (CHI3L2) ELISA Kit
The Human CHI3L2 (Chitinase 3-like protein 2) ELISA Kit is a powerful tool for accurately measuring CHI3L2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CHI3L2, also known as YKL-39, belongs to the chitinase protein family and has been implicated in various physiological and pathological processes.
It is associated with inflammation, tissue remodeling, and fibrosis, making it a valuable marker for studying conditions like asthma, rheumatoid arthritis, and cancer.By using the Human CHI3L2 ELISA Kit, researchers can gain insights into the role of CHI3L2 in disease progression and therapeutic response, ultimately paving the way for innovative treatment strategies. Trust in this kit for reliable and insightful data that can drive your research forward.
Product Name:
Human Chitinase 3 Like Protein 2 (CHI3L2) ELISA Kit
Product Code:
HUDL00561
Size:
96 Assays
Target:
CHI3L2
Synonyms:
YKL39, Chondrocyte protein 39
Detection Method:
Sandwich
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
