Human Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) ELISA Kit
The Human Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) ELISA Kit is a powerful tool for researchers studying the function of the nicotinic acetylcholine receptor alpha 5 subunit in human biology. This kit enables accurate detection of CHRNA5 levels in human serum, plasma, and cell culture supernatants with high sensitivity and specificity.CHRNA5 is an important component of the cholinergic receptor system, playing a role in various physiological functions including cognitive processes, reward mechanisms, and nicotine addiction.
Dysregulation of CHRNA5 has been linked to neurological disorders, substance abuse, and psychiatric conditions, making it a valuable target for therapeutic interventions.With its reliable and reproducible results, the Human CHRNA5 ELISA Kit is suitable for a diverse range of research applications, providing valuable insights into the role of nicotinic acetylcholine receptors in human health and disease.
Product Name:
Human Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
