Human CLIP-associating protein 2 (CLASP2) ELISA Kit
The Human CLASP2 (Clip Associating Protein 2) ELISA Kit is a cutting-edge tool designed for the precise measurement of CLASP2 levels in various biological samples, including human serum, plasma, and cell culture supernatants. Featuring unparalleled sensitivity and specificity, this kit delivers highly accurate and reproducible results, making it an indispensable asset for a wide array of research endeavors.CLASP2, also known as Clip Associating Protein 2, plays a pivotal role in cellular processes such as cell division and migration, making it a key player in a multitude of biological pathways.
Dysregulation of CLASP2 expression has been implicated in several diseases, including cancer, neurological disorders, and immune-related conditions, underscoring the significance of this protein as a potential therapeutic target. By enabling precise quantification of CLASP2 levels, this ELISA kit empowers researchers to unravel the intricate mechanisms underlying these diseases and pave the way for novel therapeutic interventions.
Product Name:
Human CLIP-associating protein 2 (CLASP2) ELISA Kit
Product Code:
HUDL00581
Size:
96 Assays
Synonyms:
Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, KIAA0627
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
