Human Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit
The Human Coiled-Coil Domain Containing Protein 80 (CCDC80) ELISA Kit is specifically designed for the accurate detection of CCDC80 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results that are ideal for a variety of research applications.CCDC80 is a protein that contains coiled-coil domains and is involved in various cellular processes, including cell adhesion and migration.
Dysregulation of CCDC80 has been implicated in several diseases, such as cancer and fibrosis, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.By utilizing the Human CCDC80 ELISA Kit, researchers can confidently measure CCDC80 levels in human samples, gaining insight into its role in disease pathology and facilitating the development of novel treatment strategies.
Product Name:
Human Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit
Product Code:
HUDL00454
Size:
96 Assays
Target:
CCDC80
Synonyms:
DRO1, SSG1, URB, Steroid Sensitive Gene 1, Down-regulated by oncogenes protein 1, Up-regulated in BRS-3 deficient mouse homolog
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
