Human Collagen and calcium-binding EGF domain-containing protein 1 (CCBE1) ELISA Kit
The Human Collagen and Calcium Binding EGF Domain Containing Protein 1 (CCBE1) ELISA Kit is specifically designed for the precise quantification of CCBE1 levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reproducible results, making it suitable for a variety of research applications.CCBE1 is a key protein that plays a critical role in the regulation of collagen and calcium binding, as well as in the growth and development of blood vessels.
Dysregulation of CCBE1 has been associated with various diseases, including lymphedema and cardiovascular disorders, making it an important target for research and potential therapeutic interventions.This ELISA kit offers researchers a valuable tool for studying the role of CCBE1 in various physiological and pathological processes, facilitating the advancement of knowledge in the field of vascular biology and the development of novel treatment strategies.
Product Name:
Human Collagen and calcium-binding EGF domain-containing protein 1 (CCBE1) ELISA Kit
Product Code:
HUDL00451
Size:
96 Assays
Synonyms:
HKLLS1, KIAA1983, Full of fluid protein homolog
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
