The Human Collagen Type XV (COL15) ELISA Kit is a reliable and accurate tool for detecting levels of collagen type XV in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research applications.Collagen type XV is a key component of the extracellular matrix, playing a crucial role in cell adhesion, migration, and tissue development. Dysregulation of collagen type XV has been linked to various pathological conditions like cancer, fibrosis, and musculoskeletal disorders, highlighting its importance as a biomarker for studying these diseases and exploring potential therapeutic interventions.
With its advanced technology and user-friendly protocol, the Human Collagen Type XV (COL15) ELISA Kit is an indispensable tool for researchers looking to delve into the intricate mechanisms of collagen biology and its implications in disease progression.
Product Name:
Human Collagen Type XV (COL15) ELISA Kit
Product Code:
HUDL00619
Size:
96 Assays
Target:
COL15
Synonyms:
COL15A1, COL15-A1, Collagen Alpha-1(XV)chain, Restin, Endostatin-XV, Related to endostatin
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
