Human Corticotropin Releasing Hormone (CRH) ELISA Kit
The Human Corticotropin-Releasing Hormone (CRH) ELISA Kit is specifically designed for the accurate and reliable detection of CRH levels in human samples. This kit offers high sensitivity and specificity, providing researchers with precise and reproducible results for a variety of research applications.CRH is a key hormone involved in the body's stress response system, playing a crucial role in regulating the release of cortisol and other stress-related hormones. Dysregulation of CRH levels has been associated with various conditions such as anxiety disorders, depression, and chronic stress-related illnesses.
Therefore, the measurement of CRH levels can provide valuable insights into the physiological and pathological processes related to stress and mental health.With its advanced technology and optimized protocols, the Human CRH ELISA Kit offers researchers a powerful tool for studying the role of CRH in health and disease. Whether investigating the mechanisms of stress response or developing potential treatments for stress-related disorders, this kit is an essential resource for those conducting research in the field of neuroendocrinology.
Product Name:
Human Corticotropin Releasing Hormone (CRH) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
