The Human CPP (Copeptin) ELISA Kit is specifically designed for the precise measurement of copeptin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it a valuable tool for a variety of research purposes.Copeptin is a peptide hormone that is released alongside vasopressin in response to stress and plays a key role in regulating water balance and blood pressure. Elevated copeptin levels have been associated with various conditions such as heart failure, sepsis, and diabetes, making it a crucial biomarker for studying these diseases and exploring potential treatment options.Overall, the Human CPP (Copeptin) ELISA Kit provides researchers with a reliable and efficient method for studying copeptin levels in human samples, offering valuable insights into its biological functions and potential clinical applications.
Product Name:
Human CPP (Copeptin) ELISA Kit
SKU:
HUES01982
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
