The Human CRP (C-reactive protein) ELISA Kit is specifically designed for the precise measurement of CRP levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for a variety of research purposes.CRP is an important biomarker of inflammation and infection in the body, with elevated levels indicating potential health issues such as cardiovascular disease, autoimmune disorders, and chronic inflammatory conditions. Monitoring CRP levels can provide valuable insights into the body's immune response and overall health status, making this ELISA kit an indispensable tool for researchers and healthcare professionals alike.
Product Name:
Human CRP (C-Reactive Protein) ELISA Kit
SKU:
HUES01330
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
