Description
Human CYP24A1 Recombinant Protein (N-His)
Human CYP24A1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln37-Gly250, with an N-terminal His tag. Predicted molecular weight: 26.81 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1) is a ~58 kDa protein. A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. For research use only.
Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol.
| Product Name: | Human CYP24A1 Recombinant Protein (N-His) |
| SKU: | GXRP02097 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CYP24A1, 24-OHase, Vitamin D(3) 24-hydroxylase, Cytochrome P450-CC24, 1, 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, CYP24, Cytochrome P450 24A1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln37-Gly250 |
| Calculated MW: | 26.81 kDa |
| UniProt: | Q07973 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
