Description
Human CYP2R1 Recombinant Protein (N-His)
Human CYP2R1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met37-Arg501, with an N-terminal His tag. Predicted molecular weight: 55.46 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Vitamin D 25-hydroxylase is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. For research use only.
Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
| Product Name: | Human CYP2R1 Recombinant Protein (N-His) |
| SKU: | GXRP11639 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CYP2R1, Cytochrome P450 2R1, EC:1.14.14.24, Vitamin D 25-hydroxylase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met37-Arg501 |
| Calculated MW: | 55.46 kDa |
| UniProt: | Q6VVX0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
