Description
Human CYP4F2 Recombinant Protein (N-His)
Human CYP4F2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly62-Ser520, with an N-terminal His tag. Predicted molecular weight: 54.89 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cytochrome P450 4F2 is a ~59 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, eicosanoids and vitamins. For research use only.
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of long- and very long-chain fatty acids. Displays high omega-hydroxylase activity toward polyunsaturated fatty acids (PUFAs). Participates in the conversion of arachidonic acid to omega-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure.
| Product Name: | Human CYP4F2 Recombinant Protein (N-His) |
| SKU: | GXRP11398 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, Arachidonic acid omega-hydroxylase, CYP4F2, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450-LTB-omega, Docosahexaenoic acid omega-hydroxylase, EC:1.14.14.1, EC:1.14.14.78, EC:1.14.14.79, EC:1.14.14.94, Leukotriene-B(4) 20-monooxygenase 1, Leukotriene-B(4) omega-hydroxylase 1, Phylloquinone omega-hydroxylase CYP4F2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly62-Ser520 |
| Calculated MW: | 54.89 kDa |
| UniProt: | P78329 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
