The Human Cytochrome c (Cyt c) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of cytochrome c levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Cytochrome c is a key component of the electron transport chain and is involved in apoptosis, the programmed cell death process. Dysregulation of cytochrome c levels has been associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring cytochrome c levels can provide valuable insights into these conditions and aid in the development of potential therapies.With its high sensitivity and specificity, the Human Cytochrome c CLIA Kit is a powerful tool for researchers studying the role of cytochrome c in health and disease. By accurately measuring cytochrome c levels, this kit can contribute to a better understanding of cellular processes and potential therapeutic targets.
Product Name:
Human Cyt-C (Cytochrome C) CLIA Kit
SKU:
HUES00054
Target:
Human Cyt-C (Cytochrome C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Cyt-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Cyt-C. You can calculate the concentration of Human Cyt-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
90-104
95-107
Average (%)
100
98
101
1:4
Range (%)
100-119
92-105
101-120
Average (%)
109
98
109
1:8
Range (%)
87-103
85-97
89-99
Average (%)
94
90
94
1:16
Range (%)
100-114
92-102
96-114
Average (%)
106
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-114
104
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.06
586.24
1766.38
184.3
557.14
1923.43
Standard deviation
19.06
69.12
138.48
23.46
57.0
160.8
C V (%)
10.47
11.79
7.84
12.73
10.23
8.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Cyt-C concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cyt-C in samples. No significant cross-reactivity or interference between Human Cyt-C and analogues was observed.
