Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit
The Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit is specifically designed for the accurate and precise measurement of COX4I1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.COX4I1 is a vital subunit of cytochrome c oxidase, a key enzyme in the mitochondrial respiratory chain involved in energy production through oxidative phosphorylation. Dysregulation of COX4I1 has been implicated in various diseases, including metabolic disorders, neurological disorders, and cancer.
Thus, accurate measurement of COX4I1 levels can provide valuable insights into mitochondrial function and its role in disease pathogenesis.Overall, the Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit is a valuable tool for researchers studying mitochondrial function, energy metabolism, and related diseases, allowing for the precise quantification of COX4I1 levels in human samples.
Product Name:
Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit
Product Code:
HUDL00644
Size:
96 Assays
Target:
COX4I1
Synonyms:
COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
