Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) ELISA Kit
The Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) ELISA Kit is specifically designed for the precise measurement of CYFRA21-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CYFRA21-1 is a fragment of cytokeratin-19, a protein found in epithelial cells, and is known to be a valuable biomarker for various cancers, particularly lung cancer.
Elevated levels of CYFRA21-1 have been associated with tumorigenesis, metastasis, and treatment response monitoring, making it a critical marker for cancer research and clinical management.With its reliable performance and ease of use, the Human CYFRA21-1 ELISA Kit is an indispensable tool for researchers and clinicians studying cancer biology, biomarker discovery, and personalized treatment strategies. Order yours today to advance your research and improve patient outcomes.
Product Name:
Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) ELISA Kit
Product Code:
HUDL00755
Size:
96 Assays
Target:
CYFRA21-1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
