The Human DAT (Dopamine Transporter) CLIA Kit is specifically designed for the precise quantification of dopamine transporter levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.The dopamine transporter is a key protein involved in the regulation of dopamine levels in the brain, playing a critical role in various neurological processes such as reward, motivation, and movement. Abnormalities in dopamine transporter function have been associated with conditions like Parkinson's disease, ADHD, and substance abuse disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.
The Human DAT CLIA Kit from Assay Genie provides researchers with a reliable tool for studying dopamine transporter biology and its implications in neurological disorders. With its high performance and user-friendly format, this kit is a valuable asset for advancing research in the field of neuroscience and drug development.
Product Name:
Human DAT (Dopamine Transporter) CLIA Kit
SKU:
HUES00701
Target:
Human DAT (Dopamine Transporter)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DAT. You can calculate the concentration of Human DAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
103-116
100-113
Average (%)
91
110
107
1:4
Range (%)
103-115
87-100
94-110
Average (%)
109
92
101
1:8
Range (%)
93-106
89-103
92-107
Average (%)
98
96
100
1:16
Range (%)
93-106
88-102
99-112
Average (%)
100
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.61
311.52
799.85
104.5
281.06
863.9
Standard deviation
12.17
30.09
90.14
8.52
23.27
71.1
C V (%)
11.52
9.66
11.27
8.15
8.28
8.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DAT in samples. No significant cross-reactivity or interference between Human DAT and analogues was observed.
