Human DCK Recombinant Protein (RPPB2496)
- SKU:
- RPPB2496
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P27707
- Research Area:
- Enzymes
Description
| Product Name: | Human DCK Recombinant Protein |
| Product Code: | RPPB2496 |
| Size: | 20µg |
| Species: | Human |
| Target: | DCK |
| Synonyms: | Deoxycytidine kinase, DCK, MGC117410, MGC138632. |
| Source: | Escherichia Coli |
| Physical Appearance: | DCK is supplied as a sterile filtered clear solution. |
| Formulation: | The DCK solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 7.5), 1mM DTT, 0.1mM PMSF, 2mM EDTA and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL |
DCK (Deoxycytidine kinase) is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. DCK is responsible for the 5`-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or UTP as phosphate donors. Deficiency of the DCK enzyme activity is linked to resistance to antiviral and anticancer chemotherapeutic agents, whereas increased DCK enzyme activity is linked to increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives.
DCK Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 296 amino acids (1-260 a.a.) and having a molecular mass of 34.6kDa. The DCK is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DCK: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Belongs to the DCK/DGK family. |
| UniProt Protein Details: | Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.74; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 4q13.3-q21.1 Cellular Component: cytosol; nucleus Molecular Function:protein homodimerization activity; nucleoside kinase activity; deoxycytidine kinase activity; drug binding; ATP binding Biological Process: pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process |
| NCBI Summary: | Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27707 |
| NCBI GenInfo Identifier: | 118447 |
| NCBI Gene ID: | 1633 |
| NCBI Accession: | P27707.1 |
| UniProt Secondary Accession: | P27707,Q5TZY7, Q6FI11, B2R8V6, |
| UniProt Related Accession: | P27707 |
| Molecular Weight: | 260 |
| NCBI Full Name: | Deoxycytidine kinase |
| NCBI Synonym Full Names: | deoxycytidine kinase |
| NCBI Official Symbol: | DCK�� |
| NCBI Protein Information: | deoxycytidine kinase; deoxynucleoside kinase |
| UniProt Protein Name: | Deoxycytidine kinase |
| Protein Family: | Deoxycytidine kinase |
| UniProt Gene Name: | DCK�� |
| UniProt Entry Name: | DCK_HUMAN |
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