Mouse M-CSF/CSF1 Recombinant Protein (RPES2314)

SKU:
RPES2314
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Mouse
€196

Description

ELISA Kit Technical Manual

Recombinant Mouse M-CSF/CSF1 Protein (Active)

Background:Macrophage colony-stimulating factor 1?M-csf?is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Name:Recombinant Mouse M-CSF/CSF1 Protein (Active)
Size:10µg
Assay Genie SKU:RPES2314
Synonyms:Macrophage colony-stimulating factor 1;CSF;MCSF;Csf1;Csfm
Species:Mouse
Expression host:Human Cells
Sequence:Lys33-Glu262
Accession:P07141
Mol Mass:26.0 kDa
AP Mol Mass:37-80 kDa?reducing condition
Bio Activity:Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.04-0.2 ng/ml.
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Cell Culture
UniProt Protein Function:M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function:protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; positive regulation of multicellular organism growth; osteoclast differentiation; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; regulation of ossification; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; positive regulation of cell migration

UniProt Code:P07141
NCBI GenInfo Identifier:1345843
NCBI Gene ID:12977
NCBI Accession:P07141.2
UniProt Secondary Accession:P07141,Q3U395, Q8R3C8,
UniProt Related Accession:P07141
Molecular Weight:29,353 Da
NCBI Full Name:Macrophage colony-stimulating factor 1
NCBI Synonym Full Names:colony stimulating factor 1 (macrophage)
NCBI Official Symbol:Csf1  
NCBI Official Synonym Symbols:op; Csfm; MCSF; C87615  
NCBI Protein Information:macrophage colony-stimulating factor 1; osteopetrosis
UniProt Protein Name:Macrophage colony-stimulating factor 1
Protein Family:C-C motif chemokine
UniProt Gene Name:Csf1  
UniProt Entry Name:CSF1_MOUSE