Description
Human DDX4 Recombinant Protein (N-His)
Human DDX4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn35-Gly131, with an N-terminal His tag. Predicted molecular weight: 12.94 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Probable ATP-dependent RNA helicase DDX4 (Vasa) is a ~79 kDa protein. ATP-dependent RNA helicase required during spermatogenesis. For research use only.
Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
| Product Name: | Human DDX4 Recombinant Protein (N-His) |
| SKU: | GXRP02935 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn35-Gly131 |
| Calculated MW: | 12.94 kDa |
| UniProt: | Q9NQI0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
