The Human DEFB (Beta-Defensin) ELISA Kit is specifically developed for the precise measurement of beta-defensin levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and dependable results, making it an excellent choice for various research purposes. Beta-defensins are key antimicrobial peptides that play a crucial role in the innate immune system, providing defense against various pathogens including bacteria, viruses, and fungi. They also have additional functions such as immune modulation and wound healing.
Understanding the levels of beta-defensins in the body can offer valuable insights into immune responses and infectious diseases. With its ability to detect and quantify beta-defensin levels effectively, the Human DEFB ELISA Kit is an indispensable tool for researchers studying immune responses, infectious diseases, and inflammatory conditions. It provides a reliable platform for exploring the role of beta-defensins in health and disease, ultimately contributing to the development of potential therapeutic strategies.
Product Name:
Human DEFB (Beta-defensin) ELISA Kit
SKU:
AEFI00440
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-100%
85-100%
85-98%
EDTA Plasma (n = 5)
86-102%
88-101%
88-97%
Heparin Plasma (n = 5)
86-103%
88-101%
86-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-104
92
EDTA Plasma (n = 5)
87-103
97
Heparin Plasma (n = 5)
85-104
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
