The Human Desmocollin-1 (DSC1) ELISA Kit from Assay Genie is a powerful tool for researchers looking to accurately measure levels of Desmocollin-1 in human biological samples. This kit is specifically designed for use with serum, plasma, and cell culture supernatants, providing researchers with a versatile and reliable option for their studies.Desmocollin-1 is a key protein involved in cell adhesion and plays a critical role in maintaining the structural integrity of tissues such as the skin and heart. Dysregulation of Desmocollin-1 has been linked to various skin disorders and cardiomyopathies, making it an important biomarker for studying these conditions and exploring potential therapeutic interventions.
The Assay Genie Human Desmocollin-1 ELISA Kit offers high sensitivity and specificity, ensuring accurate and reproducible results with every assay. Whether studying fundamental biology or investigating disease mechanisms, this kit provides researchers with the confidence to trust their data and make meaningful discoveries.Order your Human Desmocollin-1 ELISA Kit from Assay Genie today and take your research to the next level.
Product Name:
Human Desmocollin 1 (DSC1) ELISA Kit
Product Code:
HUDL00874
Size:
96 Assays
Target:
DSC1
Synonyms:
CDHF1, DG2/DG3, Cadherin family member 1, Desmosomal glycoprotein 2/3
Detection Method:
Sandwich
Range:
0.156-10 ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
