Human Dexamethasone Induced Protein (DEXI) ELISA Kit
The Human Dexamethasone-Induced Protein (DEXI) ELISA Kit is a comprehensive tool for accurately measuring DEXI levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.DEXI is a key protein that is induced by dexamethasone, a potent anti-inflammatory medication. DEXI has been implicated in various biological processes, including the regulation of immune responses and inflammation. Research suggests that DEXI may play a crucial role in conditions such as autoimmune diseases, inflammatory disorders, and cancer, highlighting its significance as a potential biomarker for studying and developing therapeutic interventions for these conditions.
The Human DEXI ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for studying DEXI levels and their implications in various disease states. With its high performance and user-friendly format, this kit is a valuable asset for advancing research in the field of immunology and inflammation.
Product Name:
Human Dexamethasone Induced Protein (DEXI) ELISA Kit
Product Code:
HUDL00821
Size:
96 Assays
Target:
DEXI
Synonyms:
MYLE, Dexamethasone Induced Transcript
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
