Description
Human DHH Recombinant Protein (N-His)
Human DHH Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu241-Tyr383, with an N-terminal His tag. Predicted molecular weight: 17.81 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Desert hedgehog protein (DHH) is a ~43 kDa protein. The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. For research use only.
Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (DHH-N). Both activities occur in the endoplasmic reticulum. Once cleaved, the C-product is degraded in the endoplasmic reticulum. Functions in cell-cell mediated juxtacrine signaling.
| Product Name: | Human DHH Recombinant Protein (N-His) |
| SKU: | GXRP00151 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DHH, Desert hedgehog protein, HHG-3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu241-Tyr383 |
| Calculated MW: | 17.81 kDa |
| UniProt: | O43323 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
