Description
Human DIAPH3 Recombinant Protein (N-His)
Human DIAPH3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp716-Phe1008, with an N-terminal His tag. Predicted molecular weight: 36.30 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein diaphanous homolog 3 (DIAPH3) is a ~136 kDa protein. Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. For research use only.
Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
| Product Name: | Human DIAPH3 Recombinant Protein (N-His) |
| SKU: | GXRP04298 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Protein diaphanous homolog 3, DRF3, DIAPH3, DIAP3, Diaphanous-related formin-3, MDia2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp716-Phe1008 |
| Calculated MW: | 36.30 kDa |
| UniProt: | Q9NSV4 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
