The Human Dicer ELISA kit from Assay Genie is a cutting-edge tool designed for the accurate measurement of Dicer levels in human biological samples. Dicer is a key enzyme involved in RNA interference and microRNA processing, playing a critical role in gene regulation and cellular function. This ELISA kit offers superior sensitivity and specificity, allowing for precise quantification of Dicer levels in serum, plasma, and cell culture supernatants. With reliable and reproducible results, researchers can confidently study the role of Dicer in various diseases and biological processes.
Dicer dysregulation has been implicated in cancer, viral infections, and neurological disorders, making it a valuable target for therapeutic intervention. By using the Human Dicer ELISA kit, scientists can gain valuable insights into the mechanisms underlying these conditions and potentially identify new treatment strategies. Overall, the Human Dicer ELISA kit from Assay Genie is an essential tool for researchers looking to deepen their understanding of RNA processing and its implications in human health and disease. Order yours today and take your research to the next level.
Product Name:
Human Dicer ELISA Kit
SKU:
HUFI00521
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-99%
86-104%
97-105%
EDTA Plasma (n = 5)
89-94%
93-100%
82-98%
Heparin Plasma (n = 5)
80-93%
83-100%
84-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-104
99
EDTA Plasma (n = 5)
86-101
94
Heparin Plasma (n = 5)
87-101
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
